Table 2. Crystallization.

Protein	SeMet ActVI-ORFA	SeMet Caci_6494	Caci_6494–DNPA	Aln2
Method	Hanging-drop vapour diffusion	Hanging-drop vapour diffusion	Hanging-drop vapour diffusion	Sitting-drop vapour diffusion
Plate type	24-well VDX	24-well VDX	24-well VDX	96-well Swissci UV-transmissible MRC 2-drop
Temperature (K)	295	295	295	295
Protein concentration (mg ml−1)	20	10	12	15
Buffer composition of protein solution	10 mM sodium succinate, 100 mM NaCl pH 5.7	10 mM MOPS pH 6.5	10 mM MOPS pH 6.5	10 mM sodium citrate pH 4.4
Composition of reservoir solution	20% PEG 3350, 0.1 M sodium citrate pH 4.5	1.47 M K2HPO4/0.07 M NaH2PO4, 5% PEG 400 pH 8.3	1.47 M K2HPO4/0.07 M NaH2PO4, 5% PEG 400, 2.5 mM (S)-DNPA pH 8.3	22% PEG 3350, 0.1 M bis-Tris, 3% ethylene glycol pH 5.5
Volume and ratio of drop	2 µl, 1:1	2 µl, 1:1	2 µl, 1:1	2 µl, 1:1
Volume of reservoir (µl)	500	500	500	50