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. 2020 Sep;1865(9):158737. doi: 10.1016/j.bbalip.2020.158737

Fig. 3.

Fig. 3

TG catabolism and proliferation of shControl/shATGL cancer cells. (A) TG hydrolase activity of shControl/shATGL cancer cells and white adipose tissue (WAT) was determined using cell/tissue lysates and a radiolabeled TG substrate. (B) TG content of shControl/shATGL cancer cells was determined after Folch extraction using a commercially available kit. (C) FA content of shControl/shATGL cancer cells was determined after Folch extraction using a commercially available kit. (D, E) Proliferation of shControl/shATGL (D) B16 and (E) CT26 cells was analyzed using a hemocytometer at the indicated timepoints. (F) Weights of shControl/shATGL allografts 14 days after cancer cell injection. Data are presented as mean values of n = 3–6 ± standard deviation. Significance was determined by student's t-test (*p ≤ 0.05, **p ≤ 0.01).