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. 2020 Sep;1865(9):158737. doi: 10.1016/j.bbalip.2020.158737

Fig. S5.

Fig. S5

Proliferation and AMPK phosphorylation of Control/ATGL-OE cancer cells. (A) Protein expression level of ATGL in Control/ATGL-OE cancer cells was analyzed by western blot analysis. GAPDH was used as loading control. (B-D) Proliferation of Control/ATGL-OE (B) C26, (C) HepG2, and (D) LLC cells was determined by seeding equal amounts of cells and counting the cells using a hemocytometer at the indicated timepoints. (E) Protein expression levels of P-AMPK (Thr172) and AMPK in Control/ATGL-OE cancer cells were analyzed by western blot analysis. GAPDH was used as loading control. (F) Relative phosphorylation level of AMPK in shControl/shATGL cancer cells was determined by densitometric quantification of immunoblots. Data are presented as mean values of n = 3–6 ± standard deviation. Significance was determined by student's t-test (*p ≤ 0.05, **p ≤ 0.01).