Table 4.

Primers and IDT PrimeTime qPCRAssay ID Used to Quantify Expression of Pituitary Genes

mRNA	Accession#	PrimeTime qPCRAssay ID	FWD	REV	Taqman Probe
Ar	NM_013476	Mm.PT.47.17416675	5’CTGCCTTGTTATCTAGCCTCA3’	5’ATACTGAATGACCGCCATCTG3’	5’ACCACATGCACAAGCTGCCTCT3’
Lhb	NM_008497	Mm.PT.45.5612498	5’CCAGTCTGCATCACCTTCAC3’	5’GAGGCACAGGAGGCAAAG3’	5’AGTACTCGGACCATGCTAGGACAGT3’
Fshb	NM_008045	Mm.PT.45.17694677	5’TTCAGCTTTCCCCAGAAGAG3’	5’TCCAGCACCAGAATAAGATGC3’	5’AGCTACGTCCTGTGCAGTCAGC3’
Cga	NM_009889	Mm.PT.58.31855537	5’CCTCAGATCGACAATCACCTG3’	5’AGCATGACCAGAATGACAGC3’	5’CCCTCAAAAAGTCCAGAGCTTGCAGA3’
Fst	NM_008046	Mm.PT.45.6344184	5’GCATTCTGGATCTTGCAACTC3’	5’GGAAGAGATAGGAAAGCTGTAGTC3’	5’CGGAAGAAACGGAGGAAGAGGAGGA3’
Gnrhr	NM_010323	Mm.PT.45.16240237	5’TCAGCATTGTCTTTGCAGGA3’	5’TCACACATTGCGAGAAGACTG3’	5’TGATCTACCTAGCAGACGGCTCTGG3’
Actb	NM_007393	Mm.PT.39a.22214843.g	5’GATTACTGCTCTGGCTCCTAG3’	5’GACTCATCGTACTCCTGCTTG3’	5’CTGGCCTCACTGTCCACCTTCC3’
Egr1	NM_007913	Mm.PT.45.13313108	5’AGCGCCTTCAATCCTCAAG3’	5’GATAACTCGTCTCCACCATCG3’	5’ATGAGCACCTGACCACAGAGTCC3’
Esr1	NM_007956	Mm.PT.47.16003033	5’CCTGTTTGCTCCTAACTTGCT3’	5’GAACCGACTTGACGTAGCC3’	5’TGCCTTCCACACATTTACCTTGATTCCT3’
Fhl1	NM_001077361	Mm.PT.58.9685318	5’GTAGCGATTCTTATAATGCACCTC3’	5’GTCACTGCTGCCTGAAGT3’	5’TTGACAAGTTCTGCGCCAACACC3’
Pgr	NM_008829	Mm.PT.47.10254276	5’CGCCATACCTTAACTACCTGAG3’	5’CCATAGTGACAGCCAGATGC3’	5’AGATTCAGAAGCCAGCCAGAGCC3’