Table 2. Concentration and soaking time of heavy-metal/halide solutions with 200 nl proteinase K crystal drops for data collected at RT.
For cryo data collection, the soaking time was about 15 min for heavy-metal soaks and 30 min for halide soaks.
| HA | Volume ejected (nl) | Stock solution (M) | Final concentration (mM) | RT soaking time (min) |
|---|---|---|---|---|
| NaBr | 5.0 | 2 | 50.00 | 15–60 |
| KBr | 5.0 | 2 | 50.00 | 25–70 |
| NaI | 5.0 | 2 | 50.00 | 10–55 |
| KI | 5.0 | 2 | 50.00 | 25–70 |
| K2Pt(NO)4 | 5.0 | 0.1 | 2.50 | 15–60 |
| KAuCl4·H2O | 2.5 | 0.1 | 1.25 | 5–45 |
| K2IrCl6 | 5.0 | 0.1 | 2.50 | 5–40 |
| SmCl3·6H2O | 5.0 | 0.1 | 2.50 | 20–55 |