Table 2. Box size and optimal defocus for different pixel sizes.

Sample	Methemoglobin (∼64 kDa)	Apoferritin (∼480 kDa)	β-Galactosidase (∼460 kDa)	70S ribosome (∼2.7 MDa)
Size (nm)	6.5	12	18	25
Pixel size (Å)	0.81/1.04/1.34	0.81/1.04/1.34	0.81/1.04/1.34	0.81/1.04/1.34
Box size (pixels)	162/126/98	298/232/180	446/348/270	618/482/374
Maximum defocus† (µm)	0.27/0.34/0.44	0.49/0.63/0.82	0.74/0.95/1.23	1.03/1.32/1.70

^†Calculated using the formula R = λΔF/d (see Section 3.2). At 300 kV, λ = 1.96876239934912 × 10⁻¹² m.