Fig. 4.

Effect of redox-modified CeO₂NP inhalation on Iba-1 immunostaining in hippocampus and cortex of 5xFAD mice.

Parasagittal brain slices of 5xFAD mice exposed to clean air of CeO₂ NPs with different doping of Zr (n = 6 per group), were stained with an antibody against Iba-1 to detect activated microglia (representative pictures are shown in A). For quantification, Iba-1 stain was determined in (B) CA1/subiculum of the hippocampus (200-fold microscopic magnification) and (C) cortex layer 5 (200-fold microscopic magnification) using image analysis software and calculated as the percentage area occupied by Iba-1 immunostaining and expressed in mean ± SEM.