Figure 1.

Effects of Short- and Long-Term Treatment with SGLT2i on Glomerular Size, Renal Injury, and Tubule-Interstitial Fibrosis

Akita mice aged 7–8 weeks were treated with and without dapagliflozin (10 mg/kg/day) for 5 days or 3 months.

(A) Immunofluorescence staining for the tubule injury marker cystatin-C at the age of 2 months.

(B and C) Representative periodic acid-Schiff (PAS) staining and quantifications of glomerular and Bowman’s space cross-sectional areas at the ages of 2 months (B) and 5 months (C).

(D and E) Immunofluorescence staining for the fibrosis marker collagen III (D) and the proximal tubule injury marker cystatin-C (E). Representative images and quantification of fluorescence intensity are shown.

(F) Representative PAS staining of the glomeruli and quantification of PAS-positive percentage of mesangial area.

Scale bar, 50 μm. Data represent the mean ± SEM of six to eight mice per group. ^∗∗p < 0.01 relative to the wild-type control group; ^##p < 0.01 relative to the untreated Akita mice group.