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. 2020 Jul 28;32(4):107954. doi: 10.1016/j.celrep.2020.107954

Figure 3.

Figure 3

Effects of High Glucose and SGLT2i on mTORC1 Activity, Glycolysis, and Mitochondrial Respiration

Cultured RPTCs (LLC-PK1 and HK2) were incubated at 5 mM glucose (low-glu) or 30 mM glucose (high-glu) with or without dapagliflozin (5 μM) for 48 h. The mTORC1 inhibitors rapamycin (rapa) and Torin-1 were used as controls.

(A and B) Immunofluorescence staining for pS6 (A) and quantification of fluorescence intensity (B) in LLC-PK1 cells.

(C) Western blotting for pS6 in LLC-PK1 cells.

(D) Immunofluorescence staining for pS6 and quantification of fluorescence intensity in HK2 cells.

(E–G) Oxygen consumption rate (OCR) (E and F) and (G) extracellular acidification rate (ECAR) in LLC-PK1 cells.

Scale bar, 50 μm. Data represent the mean ± SEM of three independent experiments. ∗p < 0.05 and ∗∗p < 0.01 relative to cells incubated at low glucose; #p < 0.05 and ##p < 0.01 relative to the high-glucose group.