Figure 4.

Effects of Constitutive Activation of mTORC1 in RPTCs on Renal Function, Albuminuria, and Interstitial Fibrosis

(A) Generation of conditional RPTC-Tsc1-KO mice.

(B and C) Western blotting (B) and immunostaining for pS6 (C). Quantification of pS6 expression is shown in (B).

(D–H) Parameters of kidney injury and function: (D) urine ACR, (E) urine KIM-1 levels, (F) serum creatinine, (G) BUN, and (H) creatinine clearance.

(I and J) Generation of renal cysts in RPTC-Tsc1-KO mice. A representative PAS staining is shown in (I) and immunofluorescence staining for pS6 in (J).

(K and L) Immunofluorescence staining and quantification of collagen III (K) and cystatin-C (L).

Scale bar, 50 μm. Data represent the mean ± SEM of four mice per group. ^∗p < 0.05 and ^∗∗p < 0.01 relative to the wild-type control group.