Figure 6.

MCL1 is an essential regulator of IEC proliferation. (A) Representative images of small intestinal or colonic tissue from i-Apc^ΔIEC and i-Apc^ΔIECMcl1^ΔIEC mice (3 days post induction), and stained with hematoxylin and eosin (H&E) or for BrdU (scale bar: 50 μm). Quantification of BrdU-positive cells per half crypt from small intestine and colon (right). Normalized expression of Pcna (B), Cdk1 (C), and Cdk4 (D) in whole small intestine tissue from wild-type controls and i-Mcl1^ΔIEC mice (4 post induction), or i-Apc^ΔIEC and i-Apc^ΔIECMcl1^ΔIEC mice (3 post induction) and analyzed by RNASeq (n = 3 per group). (E) Normalized expression of Mcl1 in whole small intestinal tissue at day 4 post induction (left) or small intestine–derived organoids at passage ≥3 (right) from wild-type or i-Mcl1^ΔIEC mice as analyzed by RNASeq (n = 3 per group). (F) Relative expression of Mcl1 in whole small intestinal tissue at day 3 post induction (left) or small intestine–derived organoids at passage ≥5 (right) in i-Apc^ΔIEC or i-Apc^ΔIECMcl1^ΔIEC mice as analyzed by RNASeq (n = 3 per group). (G) Representative images of small intestine–derived organoids from i-Apc^ΔIEC or i-Apc^ΔIECMcl1^ΔIEC mice (100x). All data presented as scatter plot graph represents mean values ± SEM. Statistical analysis was conducted by 1-tailed Mann-Whitney test where ∗P ≤ .05.