Fig. 2.

Effects of hsa_circ_0000517 down-regulation on proliferation, colony formation, cell cycle, migration, and invasion of HCC cells. a–g HCCLM3, Huh7 cells were transfected with si-hsa_circ_0000517#1, si-hsa_circ_0000517#2, or si-NC. a The expression of hsa_circ_0000517 in HCCLM3 and Huh7 cells was analyzed by qRT-PCR. b–f The proliferation, colony formation, cell cycle, migration, and invasion of HCCLM3 and Huh7 cells were analyzed via CCK-8 (b), colony formation (c), flow cytometry (d), wound healing (e), or transwell assays (f). g The protein levels of Cyclin D1, MMP2, and MMP9 in HCCLM3 and Huh7 cells were detected with western blot analysis. *P < 0.05