Fig. 4.

Proliferation, apoptosis, and cell cycle of inflammatory rDPCs after treatment with rDFSC-CM. Cells were cultured until reaching 80% confluence, and the medium was then changed to rDFSC-CM or αMEM with or without 0.5 mg/L LPS. a The proliferation of rDPCs after rDFSC-CM treatment for 1, 2, and 3 days was determined using the Cell Counting Kit-8 assay. The results are presented as the means ± SDs from at least three independent experiments. **P < 0.01. b–e The apoptosis of rDPCs was downregulated by rDFSC-CM treatment for 24 h, as detected by flow cytometry. Representative images of three independent experiments are shown. f Statistical analysis of cell apoptosis. The results are presented as the means ± SDs. **P < 0.01. g–j The cell cycle distribution of rDPC treatment with rDFSC-CM for 24 h was determined by flow cytometry. Representative images of three independent experiments are shown. k Statistical analysis of the cell cycle. The results are presented as the means ± SDs. *Compared with the αMEM group, ^#Compared with the αMEM + LPS group, **P < 0.01, ^#P < 0.05, ^##P < 0.01