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. 2020 Aug 3;8:7. doi: 10.1186/s40170-020-00214-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — ¹³C enrichments from Asp and Gln in uridine nucleotides. Nucleotides for UOK262WT (WT) and UOK252FH−/− (KO) under different conditions were quantified by IC-MS as described in the “Methods” section. An unpaired two-tailed t test was used for statistical comparisons. a Total uridine in μmole/g. UOK262 + Gln + Asn versus +Gln-Asn p = 0.148; + Gln + Asn versus –Gln + Asn p = 0.422. b, c, d¹³C fractional enrichment from ¹³C/¹⁵N Asn and ¹³C/¹⁵N glutamine in uridine, uridine monophosphate, and uridine diphosphate, respectively. Cx_Ny are isotopologues that contain x¹³C and y¹⁵N atoms. Upper: WT versus KO for ¹³C¹⁵N Asn alone and for ¹³C¹⁵N Gln alone. WT ¹³C¹⁵N Asn, KO ¹³C¹⁵N Asn, WT ¹³C¹⁵N Gln, KO ¹³C¹⁵N Gln. Lower: WT versus KO for ¹³C¹⁵N Asn with ¹²C¹⁴N (unlabeled) Gln and for ¹²C¹⁴N (unlabeled) Asn + ¹³C¹⁵N Gln. WT ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, KO ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, WT ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln, KO ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln

Inline graphic — ¹³C enrichments from Asp and Gln in uridine nucleotides. Nucleotides for UOK262WT (WT) and UOK252FH−/− (KO) under different conditions were quantified by IC-MS as described in the “Methods” section. An unpaired two-tailed t test was used for statistical comparisons. a Total uridine in μmole/g. UOK262 + Gln + Asn versus +Gln-Asn p = 0.148; + Gln + Asn versus –Gln + Asn p = 0.422. b, c, d¹³C fractional enrichment from ¹³C/¹⁵N Asn and ¹³C/¹⁵N glutamine in uridine, uridine monophosphate, and uridine diphosphate, respectively. Cx_Ny are isotopologues that contain x¹³C and y¹⁵N atoms. Upper: WT versus KO for ¹³C¹⁵N Asn alone and for ¹³C¹⁵N Gln alone. WT ¹³C¹⁵N Asn, KO ¹³C¹⁵N Asn, WT ¹³C¹⁵N Gln, KO ¹³C¹⁵N Gln. Lower: WT versus KO for ¹³C¹⁵N Asn with ¹²C¹⁴N (unlabeled) Gln and for ¹²C¹⁴N (unlabeled) Asn + ¹³C¹⁵N Gln. WT ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, KO ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, WT ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln, KO ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln