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. 2020 Aug 3;8:7. doi: 10.1186/s40170-020-00214-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — ¹³C enrichments from labeled Asp or Gln in UDPGlcNAc and metabolic subunits. N-Acetyl-D-Glucosamine 6-Phosphate and UDPGlcNAc were quantified for UOK262WT (WT) and UOK252FH−/− (KO) under different conditions by IC-MS as described in the “Methods” section. a Fractional enrichment in isotopologues of N-Acetyl-D-Glucosamine 6-Phosphate. b Total UDPGlcNAc in μmol/g protein. UOK262 + Q + N versus UOK262 + Q-N p = 0.0601; 262WT + Q + N versus +Q-N p = 0.0164, +Q + N versus –Q + N p = 0.296. c Fractional enrichment in isotopologues of UDPGlcNAc. Cx_Ny are isotopologues that contain x¹³C and y¹⁵N atoms. Upper (Panel A) and Left (Panel C): WT versus KO for ¹³C¹⁵N Asn alone and for ¹³C¹⁵N Gln alone. WT ¹³C¹⁵N Asn, KO ¹³C¹⁵N Asn, WT ¹³C¹⁵N Gln, KO ¹³C¹⁵N Gln. Lower (Panel A) and right (Panel C): WT versus KO for ¹³C¹⁵N Asn with ¹²C¹⁴N (unlabeled) Gln and for ¹²C¹⁴N (unlabeled) Asn + ¹³C¹⁵N Gln. WT ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, KO ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, WT ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln, KO ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln

Inline graphic — ¹³C enrichments from labeled Asp or Gln in UDPGlcNAc and metabolic subunits. N-Acetyl-D-Glucosamine 6-Phosphate and UDPGlcNAc were quantified for UOK262WT (WT) and UOK252FH−/− (KO) under different conditions by IC-MS as described in the “Methods” section. a Fractional enrichment in isotopologues of N-Acetyl-D-Glucosamine 6-Phosphate. b Total UDPGlcNAc in μmol/g protein. UOK262 + Q + N versus UOK262 + Q-N p = 0.0601; 262WT + Q + N versus +Q-N p = 0.0164, +Q + N versus –Q + N p = 0.296. c Fractional enrichment in isotopologues of UDPGlcNAc. Cx_Ny are isotopologues that contain x¹³C and y¹⁵N atoms. Upper (Panel A) and Left (Panel C): WT versus KO for ¹³C¹⁵N Asn alone and for ¹³C¹⁵N Gln alone. WT ¹³C¹⁵N Asn, KO ¹³C¹⁵N Asn, WT ¹³C¹⁵N Gln, KO ¹³C¹⁵N Gln. Lower (Panel A) and right (Panel C): WT versus KO for ¹³C¹⁵N Asn with ¹²C¹⁴N (unlabeled) Gln and for ¹²C¹⁴N (unlabeled) Asn + ¹³C¹⁵N Gln. WT ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, KO ¹³C¹⁵N Asn plus ¹²C¹⁴N Gln, WT ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln, KO ¹²C¹⁴N Asn plus ¹³C¹⁵N Gln