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. 2020 Aug 3;11:15. doi: 10.1186/s13227-020-00162-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The contractility and shape change of ACCs during papilla retraction. a Still images captured from live time-lapse video (one animal recorded, see Additional file 4: Video S1) of attached larva ~ 21 h post-fertilization (hpf). ACCs marked by CryBG > CD4::GFP expression. Right dorsal papilla (top arrows) retracts from 6 to 12 min timepoints, while unlabeled left dorsal papilla (bottom arrows) retracts from 18 to 24 min. b Same GFP-labeled papilla in a, but only GFP channel shown, revealing ACC contraction. c Same unlabeled papilla in a, showing the finger-like apical protrusion of the ACC retracting into papilla. f Representative image of an ACC labeled by CryBG > Unc-76::GFP at 20 hpf, indicating typical length at this stage prior to settlement. e representative image of labeled ACC at 26 hpf, indicating extreme rounded shape observed in many larvae at this stage, after settlement and metamorphosis begins. f Quantification of length:width ratio of ACCs sampled from 20 vs. 26 hpf larvae (n = 54 for 20 hpf, n = 84 for 26 hpf)