Table 1.
Site occupancies of redox PTMs for selected proteins in different functional categories.
| Protein ID | Protein Description | Subcellular Localizationa |
Cys-sitesb | Site occupancies |
||
|---|---|---|---|---|---|---|
| SSG (%) | T_oxi (%) | Log2FC | ||||
| Thioredoxin & Glutaredoxin Systems | ||||||
| THIO | Thioredoxin | N; C; S | 32#&35# | 1.5 | 9.2 | 0.3 |
| 46 | 0.8 | 2.5 | 0.2 | |||
| 73@ | 1.0 | 0.3 | ||||
| THIOM | Mitochondrial thioredoxin | M | 90#&93# | 15.8 | ||
| TRXR2 | Thioredoxin reductase 2, mito | M | 86S&9S | 2.2 | 12.9 | |
| TXND5 | Thioredoxin domain-containing (DC) protein 5 | ER | 107&114 | 82.2 | 0 | |
| 203S&206S | 4.8 | 10.1 | 0.8 | |||
| 233&240 | 23.5 | |||||
| 335S&338S | 3.9 | 11.2 | −0.3 | |||
| TXD12 | Thioredoxin DC 12 (Erp19) | ER | 64S&67S | 5.1 | 13.3 | |
| TXD17 | Thioredoxin DC 17 | C | 43#&46# | 3 | 16.6 | |
| GLRX2 | Glutaredoxin-2, mitochondrial | M; N | 70S&73S | 8.5 | 17.5 | |
| M; N | 146§ | 3.1 | 15.4 | |||
| PRDX1 | Peroxiredoxin-1 | C | 52# | 11.8 | 0.5 | |
| PRDX2 | Peroxiredoxin-2 | C | 70 | 11.8 | ||
| PRDX4 | Peroxiredoxin-4 | C; ER | 54 | 21.1 | 34.6 | 0.2 |
| PRDX5 | Peroxiredoxin-5 | M; P | 96# | 2.4 | 10.2 | 0.3 |
| PRDX6 | Peroxiredoxin-6 | C; L | 47# | 14.7 | 50.2 | 0.1 |
| Protein disulfide isomerases | ||||||
| PDIA1 | Protein disulfide-isomerase | ER; ML | 55#,58# | 4.6 | 13.2 | 1.3 |
| 314 | 2.1 | 4.2 | 0.8 | |||
| 345 | 2.8 | 0.9 | ||||
| 399S | 26.8 | 1.2 | ||||
| PDIA3 | Protein disulfide-isomerase A3 | ER; ML | 57# | 19.9 | 1.1 | |
| 60# | 14.4 | 1.1 | ||||
| 85S,92S | 3.6 | 73.9 | 0.1 | |||
| 244 | 1.6 | 8.8 | 0.7 | |||
| PDIA4 | Protein disulfide-isomerase A4 | ER; ML | 548S,551S | 10.6 | 0.6 | |
| PDIA6 | Protein disulfide-isomerase A6 | ER; ML | 291,297 | 24.0 | 0.3 | |
| Ion channels, transporters and other membrane proteins | ||||||
| AT2A2 | Sarcoplasmic/ER calcium ATPase 2 | ER | 364 | 26.0 | 29.3 | 0.5 |
| 498 | 8.2 | 6.6 | 0.4 | |||
| SO4A1 | Solute carrier organic anion transporter family member 4A1 | CM | 506S,511S,512S | 40.1 | 76.5 | 0.6 |
| AT1B3 | K-transporting ATPase subunit beta-3 | CM; ML | 144S&154S | 0.6 | 22.0 | 0.5 |
| 191S | 1.1 | 37.4 | 0.4 | |||
| 249S | 6.7 | 40.9 | 0.3 | |||
| CD68 | Macrosialin | EM; LM | 22 | 22.7 | 33.7 | 0.4 |
| 283&286S | 3.5 | 47.3 | 0.3 | |||
| ICAM1 | Intercellular adhesion molecule 1 | CM | 134 | 8.7 | 24.4 | |
| 239S | 2.4 | 21.1 | −0.1 | |||
| 292 | 33.6 | |||||
| 334 | 87.1 | |||||
| 422S | 3.6 | 51.5 | 0.3 | |||
| ITB2 | Integrin beta-2 | CM | 287S | 5.2 | 30.8 | 0.5 |
| 387 | 29.2 | 47.2 | 0.4 | |||
| 550S | 7.4 | 101.2 | 0.4 | |||
| 583S | 9.2 | 81.2 | 0.5 | |||
| 672 | 6.8 | 25.9 | 0.3 | |||
| MPRI | Cation-independent mannose-6-phosphate receptor | LM | 129S | 3.3 | 53.2 | 0.2 |
| 169S | 10.6 | 61.1 | 0.2 | |||
| 223S&230S | 8.3 | 60.1 | 0.1 | |||
| 724S | 14.8 | 61.7 | 0.3 | |||
| 823 | 24.1 | 64.6 | 0.1 | |||
| 1035 | 14.8 | 45.7 | 0.2 | |||
| 1220 | 35.3 | 97.1 | 0.0 | |||
| 1275 | 24.5 | 76.0 | 0.1 | |||
| 1469 | 8.1 | 62.9 | 0.3 | |||
| LRP1 | Prolow-density lipoprotein receptor-related protein 1 | CM; N; C | 808S | 9.0 | 49.2 | 0.4 |
| 3295S | 6.9 | 23.6 | 0.3 | |||
| 3474 | 25.4 | 64.2 | 0.4 | |||
| Other proteins | ||||||
| MT1 | Metallothionein-1 | C; L; N | 44,48,50 | 33.3 | 16.9 | 0.8 |
| MT2 | Metallothionein-2 | C; N | 33,36,41 | 16.9 | 14.6 | 0.8 |
| CATB | Cathepsin B | L; ML; EX | 93 | 10.5 | 45.0 | 0.3 |
| 105 | 2.7 | 22.4 | 0.3 | |||
| 211 | 17.5 | 71.3 | 0.3 | |||
| ENV1 | MLV-related proviral Env polyprotein | CM | 131 | 66.1 | 0.3 | |
| 162 | 83.8 | 0.4 | ||||
| 181 | 2.2 | 72.0 | 0.4 | |||
| 356S | 3.8 | 63.5 | 0.1 | |||
| GANAB | Neutral alpha-glucosidase AB | ER; G; ML | 41S &47S | 2.6 | 90.5 | 0.7 |
| 633&644 | 21.5 | |||||
| HEXB | Beta-hexosaminidase subunit beta | L | 288S | 6.6 | 62.9 | 0.7 |
| 530S | 5.9 | 50.7 | 0.5 | |||
| ICA | Inhibitor of carbonic anhydrase | S | 185&188 | 8.7 | 50.8 | 0.4 |
a
Notation for subcellular localization: C = Cytoplasm; CM = cell membrane; CS = cytoskeleton; EM = endosome membrane; ER = endoplasmic/sarcoplasmic reticulum; EX = extracellular; G = Golgi apparatus; L = lysosome; LM = lysosome membrane; M = mitochondria; ML = melanosome; N = nucleus; P = peroxisome; S = secreted.
b
# = active sites; @ = Previously reported sites of S-glutathionylation; § = binding sites (such as to metal) S = disulfide.