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Relative comparison between IgG2a and IgG1 levels in sera at weeks (A) 1, (B) 2, (C) 3, (D) 4, (E) 5, and (F) 6, respectively. LPS 10 – LPS 10 μg and immunoadjuvant 100 μg; LPS 50 - LPS 50 μg and immunoadjuvant 100 μg; NV 10 – Nanovaccine with LPS 10 μg and immunoadjuvant 100 μg; NV 50 – Nanovaccine with LPS 50 μg and immunoadjuvant 100 μg. Values are expressed as mean ± SEM. P values: *P < .05, **P < .01, ***P < .001, ****P < .0001.
