KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse CD3 | BioXCell | Cat# Clone 145–2C11, BE0001–1; RRID:AB_1107634 |
| Anti-mouse CD28 | BioXCell | Cat# Clone 37.51, BE0015–1; RRID:AB_1107624 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| MDH | Sigma Aldrich | Cat# M1567 |
| PDH | Sigma Aldrich | Cat# P7032 |
| KGDH | Sigma Aldrich | Cat# K1502 |
| MTHFD2 | Gift from Raze Therapeutics | N/A |
| [2,3,3-2H]Serine | Cambridge Isotope | Cat# DLM-582 |
| [U-13C]Glucose | Cambridge Isotope | Cat# CLM-1396 |
| [U-13C]Glutamine | Cambridge Isotope | Cat# CLM-1822 |
| [U-13C]Palmitate | Cambridge Isotope | Cat# CLM-6059 |
| [2,3,3,4,4-2H]Glutamine | Cambridge Isotope | Cat# DLM-1826 |
| [3,3,3-2H]Lactate | Cambridge Isotope | Cat# DLM-9071 |
| [U-31-2H]Palmitate | Sigma Aldrich | Cat# 366897 |
| Metformin | Sigma Aldrich | Cat# PHR 1084 |
| Phenformin | Sigma Aldrich | Cat# PHR1573 |
| Atepnin | Cayman chemicals | Cat# 119509–24-9 |
| Rotenone | Sigma Aldrich | Cat# 557368 |
| Oligomycin | Sigma Aldrich | Cat# 75351 |
| Antimycin | Sigma Aldrich | Cat# A8674 |
| Duroquinone | Sigma Aldrich | Cat# D223204 |
| UK5099 | Cayman Chemicals | Cat# 16980 |
| NAD | Sigma Aldrich | Cat# N0632 |
| NADH | Sigma Aldrich | Cat# N8129 |
| 5, 10 – meTHF | Schircks Laboratories | Cat# 16.226 |
| CoA | Sigma Aldrich | Cat# A2056 |
| Thiamine pyrophosphate | Fisher | Cat# T10835G |
| 2-hydroxypropyl-cyclodextrin | Cayman Chemicals | Cat# 16169 |
| Matrigel | Corning | Cat# 354234 |
| HT Supplement | Thermo Fisher Scientific | Cat# 11067–030 |
| Sodium Pyruvate | Gibco | Cat# 11360070 |
| Murine IL-2 | Peprotech | Cat# 212–12 |
| SHIN1 | Synthesized in house | N/A |
| SHIN2 | Synthesized in house | N/A |
| Critical Commercial Assays | ||
| Naïve CD8a+ T cell isolation kit | Miltenyi Biotec | Cat# 130–096-543 |
| Pan T cell isolation kit II | Miltenyi Biotec | Cat# 130–095-130 |
| Seahorse Mitostress Kit | Agilent | Cat# 103015–100 |
| CyQUANT NF cell proliferation assay | Thermofisher | Cat# C35006 |
| CyQUANT cell proliferation for seahorse assay | Thermofisher | Cat# C7026 |
| Experimental Models: Cell Lines | ||
| Human: HCT116 | ATCC | Cat# CCL-247; RRID:CVCL_0291 |
| Human: U2OS | Gift from Reuben Shaw (Salk Institute) | N/A |
| Human: HepG2 | ATCC | Cat# HB-8065; RRID:CVCL_0027 |
| Human: HAP1 | Horizon Discovery | N/A |
| Human: Panc1 | ATCC | Cat# CRL-1469; RRID:CVCL_0480 |
| Human: A549 | ATCC | Cat# CCL-185; RRID:CVCL_0023 |
| Human: C2C12 | ATCC | Cat# CRL-1772; RRID:CVCL_0188 |
| Human: HCT116 ΔMTHFD2 | Morscher et al., 2018 | N/A |
| Human: HCT116 ΔSHMT1 | Morscher et al., 2018 | N/A |
| Human: HCT116 ΔSHMT2 | Morscher et al., 2018 | N/A |
| Human: HEK 293T/17 | ATCC | Cat# CRL-11268; RRID:CVCL_1926 |
| Human: HEK 293T/17 ΔSHMT2 |
Morscher et al., 2018 | N/A |
| Human: HEK 293T/17 ΔSHMT2 Reexpressed | Morscher et al., 2018 | N/A |
| KrasLSL.G12D/+ x p53R172H/+ x PdxCretg/+ pancreatic tumor | Gift from Haiyong Han (TGEN) | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | Charles River Laboratories | Cat# 027; RRID:IMSR_CRL:027 |
| Mouse: NDUFS4+/− | Jackson Laboratory | Cat# 027058; RRID:IMSR_JAX:027058 |
| Mouse: CD1 nude | Charles River Laboratories | Cat# 086; RRID:IMSR_CRL:086 |
| Software and Algorithms | ||
| El-Maven | Elucidata | https://resources.elucidata.io/elmaven |
| GraphPad Prism 7 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Deposited Data | Mendeley data | http://dx.doi.org/10.17632/k27cy7fwh8.3 |
| Other | ||
| LS columns | Miltenyi Biotec | Cat# 130–042-401 |
| Pre-seperation filters (70µm) | Miltenyi Biotec | Cat# 130–095-823 |
| 6 well plates | Corning | Cat# 353046 |
| 96 well plates | Corning | Cat# 278743 |
| Seahorse XF96e | Agilent | https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers/seahorse-xfe96-analyzer |
| LC-MS Q-Exactive plus | Thermofisher | Cat# IQLAAEGAAPFALGMBDK |
| Biotek Synergy HT | Biotek | N/A |
| Mouse Jugular Vein Catheter | SAI | Cat# C20PU-MJV1301 |
| Mouse Button | Instech | Cat# VABM1B/25 |
| XBridge BEH Amide XP column | Waters | Cat# 186006725 |
| Surgery tools | Fine Science Tools | N/A |
| Cyromill | Retsch | Cat# 20.749.0001 |
| Beads | Inframat | Cat# 4039GM-S005 |
| microvette CB300 Z | Sarstedt | Cat# 16.440 |
| SolariX XR MS | Bruker | https://www.bruker.com/products/mass-spectrometry-and-separations/mrms/solarix.html |
| Just infusion Syringe Pump | Just infusion | Cat# NE-300 |
| Hypoxia Chamber | COY Laboratory | https://coylab.com/ |
| DMEM | Corning | Cat# 10–017-CV |
| RPMI | Gibco | Cat# 11875–093 |
| DMEM without glucose, glutamine, serine,glycine | US Biological | Cat# D9802–01 |
| RPMI without glucose serine/glycine | Teknova | Cat# R9660 |
| FBS | Sigma Aldrich | Cat# F2442 |
| Dialyzed FBS | Sigma Aldrich | Cat# FD392 |