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. 2020 Aug 1;2(3):zcaa013. doi: 10.1093/narcan/zcaa013

Figure 1.

Figure 1.

Effect of XRCC1 depletion and replication stress in WT cells. (A) Western blot of XRCC1 depletion by siRNA. (B) Clonogenic survival assay of XRCC1-depleted cells to ATRi. (C) Clonogenic survival assay of XRCC1-depleted cells to HU. (D) Neutral comet assay in ctrl and XRCC1 siRNA-treated cells after HU. Cells were treated with 3 mM HU for 8 h. At least 50 cells were analyzed for each experiment. (E) Relative fraction of U2OS cells positive for the DSB marker γH2AX by immunofluorescence. Cells were treated with 3 mM HU for 3 h and then allowed to recover for the indicated times. Cells were marked positive if they contained >10 foci. The number of cells analyzed in each experiment varied from 55 to 72. P-values obtained from z-tests at each time point varied from 0.88 (time 0) to 0.35 (2 h). The fractions of positive cells at time 0 were 0.49 (control siRNA, 55 cells) and 0.47 (XRCC1 siRNA, 65 cells).