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. 2020 Aug 1;2(3):zcaa013. doi: 10.1093/narcan/zcaa013

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© The Author(s) 2020. Published by Oxford University Press on behalf of NAR Cancer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — XRCC1 depletion limits fork degradation, fork restart and chromosome aberration accumulation in BRCA2-deficient cells. (A) Effect of XRCC1 depletion on replication restart in U2OS cells. (B) Effect of XRCC1 depletion on replication restart in scr-U2OS and BRCA2-U2OS cells. (C) Effect of XRCC1 depletion and MRE11 inhibition (100 μM Mirin) on replication fork protection in scr-U2OS and BRCA2-U2OS cells. At least 200 individual fibers were analyzed for each experiment. (D) Quantification of chromosome aberrations from 20 separate metaphases in scr- and BRCA2-U2OS cells with or without XRCC1 siRNA treatment. (E) Representative metaphase chromosome spread of BRCA2-U2OS with HU treatment. (F) Western blot of BRCA2 and XRCC1 co-depletion.