TABLE 1.
Comparison of glycosylation pattern between wild-type and glycosylation mutants
| Glycosylation Site | Wild-Type | Glyco-Mutant | ||
| Glycosylation (%)a | Major Glycans | Glycosylation (%)a | Major Glycans | |
| Asn-15 | 100 | Man3F | 100 | Man3F |
| Asn-51 | 78 | Man3, Man3F | Mutated | Mutated |
| Asn-80 | 100 | Man3F | Mutated | Mutated |
| Asn-140 | 66 | Man3, Man3F | 66 | Man3, Man3F |
| Asn-252 | >95 | Man7, Man8, Man9 | >95 | Man6, Man7 |
| Asn-300 | 100 | Man3F | Mutated | Mutated |
Man (oligomannose) F (fucose). In general, proteins expressed in insect cells contain high mannose N-linked oligosaccharides with varying amount of mannosyl residues; Oligoman-3 contains 3 mannosyl residues. Amino acid number corresponds to the mature protein. Man, oligomannose; F, fucose.
a
Approximate estimate.