TABLE 3.
LPL-binding characteristics of ANGPTL proteins and complexes
ANGPTL Protein or Complex | Kd (nM) | Kon (1/Ms) | Koff (1/s) |
ANGPTL3 | 343.0 | 3.2 x 104 | 1.2 × 10−3 |
ANGPTL3/8 | 6.4 | 6.9 x 104 | 4.4 × 10−3 |
ANGPTL4 | 17.7 | 9.7 x 105 | 1.7 × 10−3 |
ANGPTL4/8 | <0.001 | 4.8 x 104 | <1 × 10−7 |
The Kd, Kon, and Koff for ANGPTL3, ANGPTL3/8, ANGPTL4, and ANGPTL4/8 binding to LPL were determined using bio-layer interferometry.