FIGURE 5.

Normal and IL-6 KO mice undergo EMT associated changes at term. Western blot analysis and densitometry of mesenchymal marker vimentin in the amniotic sac of C57B/6 mice and KO IL-6 mice on day 12 (mid-gestation) and day 18 (term) of gestation. Amniotic sacs from control C57B/6 mice showed an increase in vimentin on day 18 compared to day 12 (11-fold), as previously document in CD1 models. The absence of IL-6 did not inhibit the increase in vimentin on day 18 compared to day 12 (7-fold). N = 3 for each category; mean ± SEM.