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. 2020 Aug 1;34(15-16):1075–1088. doi: 10.1101/gad.338061.120

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© 2020 Longman et al.; Published by Cold Spring Harbor Laboratory Press

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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Figure 6. — NBAS recruits UPF1 to the ER. (A) Schematic representation of the principle of fluorescence correlation spectroscopy (FCS). See the Materials and Methods for details. (B) Average autocorrelation curves of mCherry-UPF1 at the ER or cell periphery in the presence (si-control) or absence of NBAS (si-NBAS) are displayed. (C) Graph showing the derived characteristic decay times τ_D2 (slow FCS component) of mCherry-UPF1, after fitting of the autocorrelation curves with a two-component model for diffusion (plus triplet correction). Significance was determined by the two-tailed Mann-Whitney test. (****) P < 0.0001; (***) P < 0.001; (ns) not significant. (D) Average autocorrelation curves of mCherry-UPF1 at the ER with physiological (WT) or increased (GFP-NBAS) level of NBAS expression. (E) Characteristic decay times τ_D2 (slow FCS component), derived as in C of mCherry-UPF1 at the ER. Significance was determined by the two-tailed Mann-Whitney test. (****) P < 0.0001. Data showing FCS control experiments are in Supplemental Figure S8.