Skip to main content
. Author manuscript; available in PMC: 2021 Apr 30.
Published in final edited form as: Cell. 2020 Apr 20;181(3):702–715.e20. doi: 10.1016/j.cell.2020.03.051

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
HRP-conjugated anti-rabbit IgG Antibody Santa Cruz Biotechnology Cat#sc-2004
HRP-conjugated anti-mouse IgG Antibody Santa Cruz Biotechnology Cat#sc-516102
HRP-conjugated anti-goat IgG Antibody Thermo Fisher Scientific Cat#31402
normal goat IgG control R&D Systems Cat#AB-108-C
Primary antibodies for western blot, immunohistochemistry and flow cytometry, see Table S5 This paper N/A
Bacterial and Virus Strains
One Shot Stbl3 Chemically Competent E. coli Invitrogen Cat#C737303
Biological Samples
Patient-derived xenografts (PDX) T-ALL sample case 1 PROXE, DFCI Cat#MAAT-51935
Patient-derived xenografts (PDX) T-ALL sample case 2 PROXE, DFCI Cat#DFAT-28537
Patient-derived xenografts (PDX) T-ALL sample case 3 PROXE, DFCI Cat#BCAT-45575
Patient-derived xenografts (PDX) T-ALL sample case 4 PROXE, DFCI Cat#BCAT-15776
Human CD34+ HSPCs Pasquarello Tissue Bank, DFCI N/A
Chemicals, Peptides, and Recombinant Proteins
Chemicals tested in Figure 1, see Table S1 This paper N/A
Recombinant mouse IL-7 Peprotech Cat#217-17
Recombinant human IL-2 Peprotech Cat#200-02
Polyethylenimine Sigma-Aldrich Cat#408727
FuGene HD Promega Cat#E2311
Tris-HCl Sigma-Aldrich Cat#10812846001
TCEP Sigma-Aldrich Cat#C4706
PMSF Roche Cat#10837091001
Sodium chloride Sigma-Aldrich Cat#S9888
3X FLAG Peptide Millipore Cat#F4799
d-biotin IBA Cat#2-1016
Imidazole Fisher Chemical Cat#O3196-500
Propidium iodide Sigma-Aldrich Cat#P4864
RNase Thermo Fisher Scientific Cat#EN0531
Triton X-100 Sigma-Aldrich Cat#T8787
DMSO Fischer scientific Cat#BP231-100
Doxycycline Sigma-Aldrich Cat#D9891
S-adenosyl methionine Cayman Chemical Cat#13956
Formaldehyde Sigma-Aldrich Cat#252549
Critical Commercial Assays
Pierce coimmunoprecipitation kit Thermo Fisher Scientific Cat#26149
FLAG® Immunoprecipitation Kit Millipore Cat#FLAGIPT1
PP2A Immunoprecipitation Phosphatase Assay kit (modified; see Method Details) Millipore Cat#17-313
Tubulin polymerization assay > 99% pure tubulin, fluorescence-based kit CYTOSKELETON, INC Cat#BK011P
Experimental Models: Cell Lines
SUPT-13 Dr. Smith, University of Chicago, USA RRID:CVCL_9973
KOPT-K1 Dr. Nakazawa, University of Yamanashi, Japan RRID:CVCL_4965
RPMI-8402 DSMZ Cat#ACC 290, RRID:CVCL_1667
MOLT4 ATCC Cat#CRL-1582, RRID:CVCL_0013
Jurkat ATCC Cat#TIB-152, RRID:CVCL_0367
Kelly DSMZ Cat#ACC 355, RRID:CVCL_2092
BE2C ATCC Cat#CRL-2268, RRID:CVCL_0529
SKNAS ATCC Cat#CRL-2137, RRID:CVCL_1700
MHH-NB-11 DSMZ Cat#ACC 157, RRID:CVCL_1412
SHEP Maris lab, Children’s Hospital of Philadelphia, USA RRID:CVCL_0524
EBC1 Maris lab, Children’s Hospital of Philadelphia, USA RRID:CVCL_2891
NGP DSMZ Cat#ACC 676, RRID:CVCL_2141
GIMEN DSMZ Cat#ACC 654, RRID:CVCL_1232
SKNFI ATCC Cat#CRL-2142, RRID:CVCL_1702
KG1 ATCC Cat#CCL-246, RRID:CVCL_0374
MOLM13 DSMZ Cat#ACC 554, RRID:CVCL_2119
Kasumi-1 ATCC Cat#CRL-2724, RRID:CVCL_0589
OCI-AML2 DSMZ Cat#ACC 99, RRID:CVCL_1619
OCI-AML3 DSMZ Cat#ACC 582, RRID:CVCL_1844
MV4-11 ATCC Cat#CRL-9591, RRID:CVCL_0064
HEL ATCC Cat#TIB-180, RRID:CVCL_2481
SET-2 DSMZ Cat#ACC 608, RRID:CVCL_2187
THP1 ATCC Cat#TIB-202, RRID:CVCL_0006
HEK293T ATCC Cat#CRL-3216, RRID:CVCL_0063
Sf9 ATCC Cat#CRL-1711, RRID:CVCL_0549
High Five Thermo Fisher Scientific Cat#B85502, RRID:CVCL_C190
Experimental Models: Organisms/Strains
Mouse: C57BL/6 The Jackson Laboratory Cat#000664
Mouse: NSG (NOD/Scid/IL2Rgnull) The Jackson Laboratory Cat#005557
Tg(rag2:Myc; rag2:EGFP) transgenic zebrafish Look lab, DFCI, USA N/A
Casper Fish Zon lab, Boston Children’s Hospital, USA Cat#155590
Oligonucleotides
CRISPR-Cas9 target sequences: see Table S2 This paper N/A
shRNA target sequences: see Table S3 This paper N/A
PCR primers for RT-PCR: see Table S4 This paper N/A
PCR primers for gene cloning: see Table S6 This paper N/A
PCR primer: PLK1 promoter cloning forward: 5’-CCCTCTGACCAAGAAACTGAGT-3’ This paper N/A
PCR primer: PLK1 promoter cloning reverse: 5’-GCCCTTACCAAAGACACTTTCC-3’ This paper N/A
PCR primer: KIF11 promoter cloning forward: 5’-GGCGAGTGCTTACCATATATTCC-3’ This paper N/A
PCR primer: KIF11 promoter cloning reverse: 5’-AGGGCTGAGTAAACAATAGCCTAAG-3’ This paper N/A
Recombinant DNA
Plasmid: PX458 Ran et al., 2013 Addgene plasmid #48138
Plasmid: pENTR4-H1tetOx1 Dr. H. Miyoshi, RIKEN, BRC, Japan N/A
Plasmid: CS-RfA-ETBsd Dr. H. Miyoshi, RIKEN, BRC, Japan N/A
Plasmid: GFP-DRD2 Jeanneteau et al., 2004 Addgene plasmid #24099
Plasmid: qi5 Conklin et al., 1993 Addgene plasmid #24501
Plasmid: pCW57.1 Root lab, Broad Institute, MIT. Addgene plasmid #41393
Plasmid: pENTR1A dual selection vector Thermo Fisher Scientific Cat#A10462
Plasmid: CSII-EF-MCS-IRES2-Venus Dr. H. Miyoshi, RIKEN, BRC, Japan N/A
Plasmid: CSII-EF-MCS-IRES2-hKO1 Dr. H. Miyoshi, RIKEN, BRC, Japan N/A
Plasmid: PathDetect pSRE-Luc Cis-Reporter Agilent Technologies Cat#219080
Plasmid: pMD2.G Trono lab, EPFL, France Addgene plasmid #12259
Plasmid: psPAX2 Trono lab, EPFL, France Addgene plasmid #12260
Plasmid: pAC8RedNK Abdulrahman et al., 2009 N/A
Plasmid: pGL4.20 Promega Cat#E6751
Plasmid: pCDNA3 WT B-Myb Johnson et al., 1999 Addgene Plasmid #25965
Plasmid: pRL-CMV Promega Cat#E2261
Software and Algorithms
GraphPad Prism (version 7) GraphPad RRID: SCR_002798
Microsoft Excel Microsoft RRID: SCR_016137
ImageJ (version 1.49u) Schneider et al., 2012 https://imagej.net/Welcome
FlowJo (version 10.6.1.) FlowJo, LLC RRID:SCR_008520
Other
Q5 Site-Directed Mutagenesis Kit New England BioLabs Cat#E0554S
cOmplete Mini EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich Cat#11836170001
Strep-Tactin XT Superflow IBA GMBH Cat#2-4012-001
Ni Sepharose 6 Fast Flow affinity resin GE Healthcare Cat# GE17-5318-01
RNeasy Mini Kit QIAGEN Cat#74104
PrestoBlue Cell Viability Reagent Thermo Fisher Scientific Cat# A13261
SpectraMax M5 Microplate reader Molecular Devices LLC Cat#M5
SuperSignal West Femto Maximum Sensitivity substrate Thermo Fisher Scientific Cat#34095
ImageQuant LAS4000 GE Healthcare Life Sciences Cat#28955810
RPMI 1640 Medium Thermo Fisher Scientific Cat#11875093
Alpha-minimum essential media 1x Glutamax-1 GIBCO Cat#32571-036
Human AB serum Sigma Cat#H4522
Insulin-Transferrin-Selenium GIBCO Cat#41100-045
CD34 MicroBead Kit, human Miltenyi Biotec Cat#130-046-702
StemMACS HSC Expansion Media XF Miltenyi Biotec Cat#130-100-473
StemMACS HSC Expansion Cocktail, human Miltenyi Biotec Cat#130-100-843
Amaxa 4D-Nucleofector Lonza Cat#AAF-1002B
Multiple Lentiviral Expression System Kit Albers et al., 2015 Addgene Kit # 1000000060
FACS cell sorter BD Biosciences Cat#BD FACSAria III
ReverTra Ace® qPCR RT Master Mix TOYOBO Cat#FSQ-201
ESF 921 Insect Cell Culture medium Expression Systems Cat#96-001-01
SF-900 II SFM medium Thermo Fisher Scientific Cat#10902088
ANTI-FLAG M2 affinity gel Sigma-Aldrich Cat#A2220
SuperBlue Ultra Coomassie stain Protea Biosciences Cat#SB-G250X-KIT
FACS analyzer BD Biosciences Cat#BD FACS LSR II
FACS analyzer BD Biosciences Cat# BD LSRFortessa
Dual-Luciferase® Reporter Assay System Promega Cat#E1910
FITC Annexin V Apoptosis Detection Kit with PI BioLegend Cat#640914
BSA Sigma-Aldrich Cat#A3311
DMEM - Dulbecco’s Modified Eagle Medium Thermo Fisher Scientific Cat#11960069
Acetocarmine solution TCI Chemicals Cat#A0050
Fluorescence Microscope Carl Zeiss Microscopy GmbH Cat#Zeiss Axio Observer Inverted Fluorescence Microscope
PBS Thermo Fisher Scientific Cat#10010023
SILAC Protein Quantitation Kit (LysC), RPMI 1640 Thermo Fisher Scientific Cat#A33971
Pierce Mass Spec Sample Prep Kit for Cultured Cells Thermo Fisher Scientific Cat#84840
High-Select TiO2 Phosphopeptide Enrichment Kit Thermo Fisher Scientific CatA32993
FBS Thermo Fisher Scientific Cat#16000044