Table 4.
IC50s calculated for the isolated compounds from Artemisia campestris. in the viral transcription assay. MT–2 cells were transfected with a luciferase plasmid (pNL.43–Luc.1 ng/106 cells) under the control of the whole genome of the wild type HIV and treated with serial dilutions of damsin, canrenone, cardamonin, acerosin, trimethoxyflavone and xanthomicrol. Results were obtained using the luciferase assay system following the manufacturer instructions after 48 h in culture measuring the RLUs in a luminometer. 100% was the RLUs obtained by untreated controls. IC50 was calculated using Prism v8.0 (GraphPad Software) using non–linear regression, dose–response curves. CI95%: Confidence interval 95%.
| IC50 μM HIV (CI95%; R2) | |
|---|---|
| Damsin | 0.13 (0.097–0.18; 0.9926) |
| Canrenone | 9.18 (6.74–12.49; 0.9932) |
| Cardamonin | >100 |
| Acerosin | >20 <100 |
| Trimethoxyflavone | 0.64 (0.4451–0.9336; 0.9897) |
| Xanthomicrol | 18.06 (4.101–216.5; 0.8110) |