Table 5.
EC50 calculated for the isolated compounds from Artemisia campestris. in the glutathione assay. Total, reduced (GSH) and oxidized glutathione (GSSG) levels were determined in THP–1 cells extracts. Fluorimetric measurement of the interaction of thiols with OPT (o–Phtaldehyde) in the presence of serial dilutions of damsin, canrenone, cardamonin, acerosin, trimethoxyflavone, xanthomicrol and resveratrol (control) were compared to a standard curve produced by measuring increasing concentrations of GSH. Fluorescence was measured at λexc = 350 nm and λem = 420 nm. The redox index (RI), a parameter that indicates the antioxidant status of the tissue, was expressed as follows: RI = GSH/(GSH + GSSG). Results are expressed as EC50 (concentration that produce the 50% of the maximum antioxidant effect), being the maximum 1 (all of the glutathione is in the reduced form). Besides, we have also calculated the Emax reached by each compound to evaluate antioxidant efficacy (Emax). Both parameters were calculated using Prism v8.0 (GraphPad Software) using non–linear regression, dose–response curves.
| EC50 μM HIV (CI95%; R2) | Emax (CI95%) | |
|---|---|---|
| Damsin | > 100 | – |
| Canrenone | > 100 | – |
| Resveratrol | 1.85 (1.56–2.19; 0.9923) | 0.94 (0.92–0.96) |
| Cardamonin | 5.39 (1.03–28.28; 0.8782) | 0.84 (0.55–1.13) |
| Acerosin | 4.58 (2.76–7.61; 0.9636) | 0.95 (0.88–1.02) |
| Trimethoxyflavone | 3.79 (0.42–34.13; 0.776) | 0.74 (0.47–1.00) |
| Xanthomicrol | 4.59 (2.03–10.36; 0.9209) | 0.93 (0.81–1.04) |