Table 1.
Comparison of luciferases assayed in oral streptococci
| Luciferase | Substrate | Emission (nm) |
ORF (bp) |
ATP | Source organism | Comments |
|---|---|---|---|---|---|---|
| Click beetle greena | d-Luciferin | 537 | 1629 | Yes | Pyrophorus plagiophthalamus | Less bright compared to Luciola red |
| Cypridinaa | Vargulin | 460 | 1611 | No | Vargula hilgendorfii | Very bright, currently suitable for in vitro use only |
| Firefly | d-Luciferin | 557 | 1653 | Yes | Photinus pyralis | Yellow-green color is not suitable for multiplexing |
| Green Renillaa | Coelenterazine | 527 | 945 | No | Renilla reniformis | Very bright, suitable for multiplexing |
| Luciola reda | d-Luciferin | 609 | 1647 | Yes | Luciola italica | Suitable for multiplexing |
| Renilla | Coelenterazine | 420 | 936 | No | Renilla reniformis | Very bright, poorer tissue penetration than Green Renilla |