Fig. 4.

Suppression of PDCD6 expression attenuates MAPK signaling pathway activity. a. A heatmap of differentially expressed genes in PDCD6-knockdown and control HCT15 cells that were generated using R software. The log₂ values were calculated for each sample by normalizing the data to the number of reads alone (P < 0.05). b. The functional category based on gene ontology (GO) term enrichment. c. The signaling pathway based on KEGG enrichment analysis of signal transduction pathway sub-categories. d. Functionally grouped networks based on KEGG pathway analysis. The genes are presented for the PDCD6-KD and control cells. e. Gene set enrichment analysis (GSEA) of genes involved in cell proliferation in HCT15 PDCD6 knock-down cells with the gene sets corresponding to the MAPK signaling pathway in the KEGG database (NES Score = 0.05 and FDR = 0.10). f. A heatmap, which was generated using R software, of MAPK signaling pathway-related genes that were differentially expressed in HCT15 vector control and PDCD6 knock-down cells using R software. The log₂ values were calculated for each sample by normalizing the data to the number of reads alone (p < 0.05 and FDR < 0.05). g. Quantitative real-time PCR analysis of the relative mRNA expression of MYC, JUN, DUSP5, GADD45B, PTPN7, NFATC1, RASGFR2, PLA2G4A, MECOM and PDCD6 in control and PDCD6-knockdown HCT15 cells normalized to actin expression (means± SDs, t-test, and *, p < 0.05). The experiments were repeated three times