Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Aug 3;39:148. doi: 10.1186/s13046-020-01650-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 6 — FAK or Src inhibitor selectively inhibits the cell viability of HCC cells with high expression of COL4A1. a&b Cell viability was tested after treatment with inhibitors. HCC cells were treated with Defactinib (FAK inhibitor) or Saracatinib (Src inhibitor) at the indicated concentrations for 48 h. HCC cells with high expression level of COL4A1 (a) were sensitive to Defactinib or Saracatinib treatment, but HCC cells with low expression level of COL4A1 (b) were resistance to Defactinib or Saracatinib treatment. c&d Knockdown of COL4A1 reduced the sensitive to Defactinib or Saracatinib treatment in SMMC7721 (c) and SK-Hep1 (d). Indicated cells were treated with Defactinib (FAK inhibitor) or Saracatinib (Src inhibitor) at the indicated concentrations for 48 h. e&f Overexpression of COL4A1 increased the sensitive to Defactinib or Saracatinib treatment in HepG2 (e) and PLC/PRF/5 (f). Indicated cells were treated with Defactinib (FAK inhibitor) or Saracatinib (Src inhibitor) at the indicated concentrations for 48 h. Cell viability was analyzed by crystal violet staining assay (Left) and CCK8 assay (Right), respectively. Data are presented as means ± standard deviation. Student t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant