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. 2020 Aug 2;8(1):e001113. doi: 10.1136/bmjdrc-2019-001113

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© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Reductions in fibrotic pathway activation dominate the transcriptomic response to RYGB. (A) Principal component analysis-based summarization of the renal cortical transcriptome in fa/+, SHAM and RYGB-operated rats. Volcano plot visualization of differential expression analysis for the SHAM versus fa/+ comparison (B) and RYGB versus SHAM comparison (C). Reactome-based analysis of pathway level changes in the SHAM versus fa/+ (D) and RYGB versus SHAM comparisons (E). (F) Alignment of pathway level changes between SHAM versus fa/+ and RYGB versus SHAM comparisons indicating directionality of change. A fold-cut off change of 1.3 was applied in pathway analyses. In each graphic, adjusted p value and gene ratios for each significantly altered pathway are represented as a function of color and size of dots, respectively. (G) Heatmap visualization of Microenvironment Cell Populations (MCP) counter-based estimates of the abundance of eight immune and two stromal cell populations. Data are overlaid with heatmap annotation of quantitative measures of glomerular structure and ultrastructure for each sample included in the transcriptomic analysis. Color coding indicates per sample relative abundance as a function of centered and scaled row-specific data. Blue indicates low relative abundance and orange-red increasing relative abundance. (H) Representative images of Picrosirius Red staining for total collagen in renal cortex. GBM, glomerular basement membrane; PFPD, podocyte foot process diameter; PFPF, podocyte foot process frequency; RYGB, Roux-en-Y gastric bypass; SHAM, sham surgery (laparotomy).