KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD133/2-APC | Miltenyl Biotec | 130-098-129 |
| CD31-APC-Cy7 | BioLegend | 303119 |
| HPi2-Alexa-405 | Novus Biologicals | NBP1-18946AF405 |
| HPx1-Alexa-647 | Novus Biologicals | NBP1-18951AF647 |
| ChromPure Mouse IgG | Jackson ImmunoResearch | 015-000-003 |
| CD133/1-APC | Miltenyl Biotec | 130-113-668 |
| Biological Samples | ||
| Human pancreatic samples | Albeta Diabetes Institute IsletCore | |
| Critical Commercial Assays | ||
| KAPA HiFi HotStart ReadyMix | KAPA Biosystems | KK2601 |
| Nextera XT | Illumina | FC-131-1096 |
| LIVE/DEAD Fixable near-IR dead cell dye | Life Technologies | L10119 |
| ERCC spike-in control | ThermoFisher | 4456740 |
| TRIzol Reagent | ThermoFisher | 15596018 |
| 5X All-In-One RT Master Mix | Applied Biological Materials (abm) Inc. | G486 |
| Invitrogen ultrapure distilled water | ThermoFisher | 10977-015 |
| 10% Triton | Sigma-Aldrich | 93443 |
| Recombination RNase inhibitor | Clontech | 2313A |
| SUPERase-In RNase Inhibitor | ThermoFisher | AM2696 |
| 10 mM dNTP | ThermoFisher | 18427088 |
| DharmaFECT 1 | Dharmacon | T-2001-03 |
| Fast SYBR Green Master Mix | ThermoFisher | 4385612 |
| DMEM medium | ThermoFisher | 11885092 |
| All chemicals | Sigma-Aldrich | |
| Deposited Data | ||
| Single cell mRNA-seq data | Enge et al., 2017 | GEO:GSE81547 |
| Single cell mRNA-seq data | Segerstolpe et al., 2016 | ENA:ERP017126, ArrayExpress: MTAB-5060 |
| Single cell mRNA-seq data | This paper | GEO:GSE124742 |
| Processed patch-seq datasets | This paper | https://github.com/jcamunas/patchseq |
| Oligonucleotides | ||
| SmartSeq2 OligodT: 5′–AAGCAGTGGTATCAACGCAGAGTACT30VN-3′ | Picelli et al., 2014 | |
| SmartSeq2 TSO: 5′-AAGCAGTGGTATCAACGCAGAGTACATrGrG3′ | Picelli et al., 2014 | |
| SmartSeq2 ISPCR: 5′-AAGCAGTGGTATCAACGCAGAGT-3′ | Picelli et al., 2014 | |
| siRNA (OGDHL, TSPAN1, FAM159B, RGS9, GYG1, ETV1) - see Method Details | ThermoFisher Scientific | IDs # s31422, s19659, s264018, s16736, s6360, s4855 |
| scramble siRNA | Qiagen | 1027284 |
| Primers for qPCR (OGDHL, TSPAN1, FAM159B, RGS9, GYG1, ETV1) - see Supp. Table S4 | Integrated DNA Technologies (IDT) | |
| Software and Algorithms | ||
| STAR | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| HTSeq | Anders et al., 2015 | https://github.com/simon-anders/htseq |
| Scanpy | Wolf et al, 2019 | https://github.com/theislab/scanpy |
| Custom analysis software | This paper | https://github.com/jcamunas/patchseq |
| PatchMaster software (recording) | HEKA Elektronik | |
| Fitmaster software (analysis) | HEKA Elektronik | |
| Sigmaplot software (analysis) | SYSTAT | |