Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 24;152(8):e201912515. doi: 10.1085/jgp.201912515

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Ridone et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Colocalization of PIEZO1-GFP and CtxB raft marker using TIRF. (A) TIRF microscopy of cholesterol-rich domains labeled with CtxB–Alexa Fluor 555 (A555; red) on the basal membrane of PIEZO1-GFP–expressing HEK293T cells (GFP fluorescence in green). (B) Average number of PIEZO1-GFP entities per square micrometer detected after 30-min treatments. (C) Average density of colocalized entities per square micrometer detected after 30-min treatments. Control (n = 6 trials, 42 cells), MBCD (n = 6 trials, 81 cells), cholesterol (n = 6 trials, 62 cells), and dynasore (n = 6 trials, 55 cells). (D) Effective diffusion rate of colocalized PIEZO1-GFP/CtxB–A555 at the micrometer scale, before and after treatments. Examples of STICCS correlation function time courses are shown in Video 1. One-way ANOVA; ***, P < 0.0005; ****, P < 0.0001; error bars represent SEM. Scale bars represent 5 µm.