Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 24;152(8):e201912515. doi: 10.1085/jgp.201912515

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Ridone et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 6. — Colocalization between PIEZO1-GFP and Laurdan phases. (A) Fluorescence microscopy of hP1-1591-CL cells labeled with Laurdan. PIEZO1-GFP (red) is tested for colocalization with either the liquid-ordered phase or the liquid-disordered signals emitted by the Laurdan dye. The images on the left column display the signal emitted by ordered-phase Laurdan (green signal, emission 400–460 nm) and the right column shows the signal emitted by disordered-phase Laurdan (cyan signal, emission 470–530 nm). Scale bars represent 10 µm. The inset on the top right shows a magnified portion of each control image (dashed white box) and highlights the difference in signal intensity and degree of colocalization with PIEZO1-GFP exhibited by either membrane phase labeled by the Laurdan dye. (B) Density of PIEZO1-GFP entities per square micrometer detected under various conditions. (C) Density of ordered Laurdan entities. (D) Density of disordered Laurdan entities. (E) Density of colocalized PIEZO-GFP/ordered Laurdan entities as determined by image correlation spectroscopy. (F) Density of colocalized PIEZO-GFP/disordered Laurdan entities. n = 1 represents data obtained from a single cell. Control PIEZO1-GFP (n = 31 cells), 5 mM MBCD (n = 32 cells), 50 µg/ml cholesterol (n = 31 cells), 80 µM dynasore (n = 31 cells). Statistical analysis was performed using one-way ANOVA; *, P < 0.05; ****, P < 0.0001; error bars represent SEM.