Table 2. Summary of average P50 values and slopes derived from Boltzmann fits of I/Imax versus pressure plots of all PIEZO1 variants and treatments performed in this study.
| P50 (−mmHg) | Slope of Boltzmann α−1 (mmHg−1) | ∆G (kBT) | |
|---|---|---|---|
| hPIEZO1-GFP | 36.4 ± 3 | 14 ± 2.5 | 3.5 ± 0.4 |
| + 0.1 mM MBCD | 30.7 ± 7 | 13.3 ± 1.3 | 3.2 ± 0.5 |
| + 1 mM MBCD | 47.1 ± 0.6 | 8.3 ± 3.7 | 5.7 ± 1.3 |
| + 5 mM MBCD | 54.2 ± 1.1 | 13.3 ± 1 | 5.3 ± 1.2 |
| + 100 µg/ml cholesterol | 36.8 ± 2.5 | 14.4 ± 2 | 3.5 ± 0.2 |
| + 80 µM dynasore | 36.7 ± 4.8 | 16.3 ± 3.9 | 3.7 ± 1 |
| + 25 µM DHA (24 h) | 16 ± 2.8 | 12.3 ± 1.4 | 1.6 ± 0.4 |
| + 25 µM DHA (48 h) | 27 ± 1.8 | 10.3 ± 1.4 | 3.6 ± 0.8 |
| hPIEZO1 WT | 39.6 ± 2.1 | 11.6 ± 2 | 4.4 ± 0.5 |
| + 5 mM MBCD | 42.2 ± 2.6 | 14.3 ± 2.1 | 3.7 ± 0.6 |
| hPIEZO1 R2456H | 13.3 ± 7 | 15.1 ± 3 | 1.3 ± 0.6 |
| + 5 mM MBCD | 16.4 ± 5.6 | 14.1 ± 2.8 | 1.9 ± 0.5 |
| N2A mPIEZO1 | 17.8 ± 2.2 | 6.6 ± 1.6 | 4.7 ± 0.3 |
| + 5 mM MBCD | 22.5 ± 1.8 | 8.4 ± 1.7 | 4 ± 0.7 |
Gating free energy calculations were estimated following Eq. 7 (∆G/kBT ≈ P50 ∙ α) in Hamill and Martinac (2001). Statistical difference between ∆G detected for PIEZO1-GFP versus 25 µM DHA (24 h), P = 0.0153, Student’s t test. All values are reported as mean ± SEM.