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. 2020 May 22;152(8):e201912525. doi: 10.1085/jgp.201912525

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© 2020 Mansley et al.

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Figure 9. — Transcripts for EP receptors and for enzymes of the PGE₂ biosynthetic pathway were detected in mCCD_cl1 cells. Specific primers were used to detect the transcript expression of the prostanoid receptors EP₁–EP₄ (A), as well as key enzymes involved in PGE₂ synthesis, namely COX-1, COX-2, mPGES1, mPGES2, and cPGES (B). Transcript expression was determined in RNA extracted from mCCD_cl1 cells, as well as from mouse cortical kidney and lung homogenates. H₂O and primers recognizing β-actin served as no-template and loading control, respectively. Expected individual transcript sizes are indicated by open arrowheads.