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. Author manuscript; available in PMC: 2021 Feb 24.
Published in final edited form as: Dev Cell. 2020 Feb 6;52(4):446–460.e5. doi: 10.1016/j.devcel.2020.01.006

Figure 5. Rho-GTPases drive Yap mechanotransduction and HSPC production in vivo.

Figure 5.

(A) WISH for runx1/cmyb at 36hpf in WT embryos after treatment (12–36hpf) with Rho-GTPase activators CN02, CN03 and CN04. Scale bar is 100μm.

(B) Distribution plot of embryos in 5A (n≥50/condition, Chi-squared test, *p<0.05, ***p<0.001).

(C) qPCR after treatment with CN02 or CN04 (unpaired Student’s t-test versus control, *p<0.05, **p<0.01, ***p<0.001. Error bars indicate S.E.M.).

(D) Quantification of Cd41:GFP+ cells in images of the CHT at 72hpf (n=30 control; 23 CN02; 22 CN04, unpaired Student’s t-test, ***p<0.001, Error bars indicate S.D.).

(E) WISH for runx1/cmyb at 36hpf in sih morphants after CN02 treatment (12–36hpf). Scale bar is 100μm.

(F) Distribution plot of embryos in 5F (n≥50/condition, Chi-squared test, ***p<0.001).

(G) qPCR in WT embryos and sih morphants at 36hpf after CN02 treatment (unpaired Student’s t-test, **p<0.01, ***p<0.001. Error bars indicate S.E.M.).