| Antibodies |
| CD34-PE Cy7 |
BD Biosciences |
Cat. 348791 |
| CD43-PE |
BD Biosciences |
Cat. 560199 |
| CD45-PE Cy5 |
Beckman Coulter |
Cat. IM2652U |
| CD235a-FITC |
BioLegend |
Cat. 349104 |
| VEGFR2-APC |
BD Biosciences |
Cat. 560495 |
| DAPI solution |
BD Biosciences |
Cat. 564907 |
| Monoclonal YAP1 |
Santa Cruz Biotechnology |
Cat. sc-101199 |
| Monoclonal GAPDH |
Santa Cruz Biotechnology |
Cat. sc-47724 |
| Alexa Fluor 488 Phalloidin |
Invitrogen |
Cat. A12379 |
| Alexa Fluor 647-AffiniPure donkey anti-mouse IgG |
Jackson ImmunoResearch Laboratories |
Cat. 715-605-150 |
|
|
|
| Biological Samples |
|
|
| Cord Blood CD34+ Cells |
AllCells |
Cat. CB008F |
| mPB CD34+ Cells |
AllCells |
Cat. mPB015F |
| Mouse Embryonic Fibroblasts |
GlobalStem |
Cat. 6001G |
| Primary Human Umbilical Vein Endothelial Cells |
ThermoFisher |
Cat. C0155C |
|
|
|
| Chemicals, Peptides, and Recombinant Proteins |
| CN02: Rac/Cdc42 Activator II |
Cytoskeleton, Inc |
Cat. CN02 |
| CN03: Rho Activator II |
Cytoskeleton, Inc |
Cat. CN03 |
| CN04: Rho/Rac/Cdc42 Activator I |
Cytoskeleton, Inc |
Cat. CN04 |
| CT04: Rho Inhibitor I |
Cytoskeleton, Inc |
Cat. CT04 |
| CHIR99021 |
StemCell Technologies |
Cat. 72052 |
| SB431542 |
StemCell Technologies |
Cat. 72232 |
| Recombinant Human BMP4 |
PeproTech |
Cat. 10779-138 |
| Recombinant Human bFGF |
Gibco |
Cat. PHG0266 |
| Recombinant Human VEGF |
R&D Systems |
Cat. 293-VE |
| Recombinant Human IL6 |
PeproTech |
Cat. 200-06 |
| Recombinant Human IL11 |
PeproTech |
Cat. 200-11 |
| Recombinant Human IGF1 |
PeproTech |
Cat. 100-11 |
| Recombinant Human SCF |
PeproTech |
Cat. 300-07 |
| Recombinant Human EPO |
Epogen |
Cat. 55513-144-01 |
| Recombinant Human TPO |
PeproTech |
Cat. 300-18 |
| Recombinant Human Flt3L |
PeproTech |
Cat. 300-19 |
|
|
|
| Critical Commercial Assays |
| Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye |
Invitrogen |
Cat. C10340
|
| TSA Plus Cyanine 3/Fluorescein Kit |
Perkin Elmer |
Cat. NEL753001KT |
| Experimental Model: Cell Lines |
| CD45-iPSC |
BCH iPS Core |
Vo et al., 2018 |
| MSC-iPSC1 |
BCH iPS Core |
Park et al., 2008 |
| 1157-iPSC |
BCH iPS Core |
Sugimura et al., 2017 |
| Dub7-iPSC |
Kaufman Laboratory, UCSD |
Ferrell et al., 2015 |
| Experimental Model: Zebrafish |
| Ctgfa:GFP: Tg2(ctgfa:EGFP)pd96
|
|
Mokalled et al., 2016 |
| Hs:YapS87A: Tg(hsp70:mCherry-2A-Flag-YapS87A)
|
|
Cox et al., 2018 |
| Yap−/−: yap1mw48/mw48
|
|
Miesfeld et al., 2015 |
| Flk1:GFP: Tg(kdrl:gfp)la116
|
|
Choi et al., 2007 |
| Kdrl:mCherry: Tg(kdrl:Hsa.HRAS-mCherry)s896
|
|
Chi et al., 2008 |
| Kdrl:mCherry: Tg(kdrl:Hsa.HRAS-mCherry)s916
|
|
Hogan et al., 2009 |
| Cd41:GFP: Tg(−6.0itga2b:egfp)la2
|
|
Bertrand et al., 2008 |
|
|
|
| Oligonucleotides |
| Standard control morpholino |
CCTCTTACCTCAGTTACAATTTATA |
GeneTools |
|
lats1 morpholino |
CCTCGGGTTTCTCGGCCCTCCTCAT |
Chen et al., 2009 |
|
lats2 morpholino |
CATGAGTGAACTTGGCCTGTTTTCT |
Chen et al., 2009 |
|
sih morpholino |
CATGTTTGCTCTGATCTGACACGCA |
Sehnert et al., 2002 |
| hLATS1 siRNA |
ThermoFisher |
Cat. s17393 |
| hLATS2 siRNA |
ThermoFisher |
Cat. s25503 |
| Negative Control siRNA |
ThermoFisher |
No 1 |
| runx1_fw |
CGTCTTCACAAACCCTCCTCAA |
Carroll et al., 2014 |
| runx1_rv |
GCTTTACTGCTTCATCCGGCT |
Carroll et al., 2014 |
| cmyb_fw |
TGATGCTTCCCAACACAGAG |
Carroll et al., 2014 |
| cmyb_rv |
TTCAGAGGGAATCGTCTGCT |
Carroll et al., 2014 |
| kdr1_fw |
CGAACGTGAAGTGACATACGG |
Carroll et al., 2014 |
| kdr1_rv |
CCCTCTACCAAACCATGTGAAA |
Carroll et al., 2014 |
| ctgfa_qPCR_fw |
CTACGGCTCCCCAAGTAACC |
Cox et al., 2016 |
| ctgfa_qPCR_rv |
TCCACTGCGGTACACCATTC |
Cox et al., 2016 |
| ctgfa_ISH_fw |
TGTGATTGCTCTGCTGTTCC |
Mokalled et al., 2016 |
| ctgfa_ISH_rv |
GGTGAGGCGATTAGCTTCTG |
Mokalled et al., 2016 |
| 18s_fw |
TCGCTAGTTGGCATCGTTTAT |
Esain et al., 2015 |
| 18s_rv |
CGGAGGTTCGAAGACGATCA |
Esain et al., 2015 |
| yap gen FWD |
AGTCATGGATCCGAACCAGCACAA |
Cox et al., 2018 |
| yap gen REV |
GCAGGCTGAAAGTGTGCATTGCC |
Cox et al., 2018 |
| ANKRD1 |
ThermoFisher |
Hs00923599_m1 |
| CTGF |
ThermoFisher |
Hs00170014_m1 |
| LATS1 |
ThermoFisher |
Hs01125524_m1 |
| LATS2 |
ThermoFisher |
Hs01059009_m1 |
| CD43 |
ThermoFisher |
Hs01058311_m1 |
| CD45 |
ThermoFisher |
Hs04189704_m1 |
| RUNX1 |
ThermoFisher |
Hs00231079_m1 |
| 18s |
ThermoFisher |
Hs99999901_s1 |
|
|
|
| Recombinant DNA |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
| Software and Algorithms |
