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. 2020 Jul 20;217(8):e20192336. doi: 10.1084/jem.20192336

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© 2020 Ruscher et al.

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Figure 2. — Beneficial conditions for CD8αα IEL establishment in early life. (A) Experimental outline: thymic DN cells from donor mice were adoptively transferred into congenically different 15-d-old or adult recipients. Establishment of donor-derived IELs was determined 4–5 wk later by flow cytometry. (B and C) Number of donor-derived small intestinal CD8αα IELs (B) or TCRγδ⁺ IELs (C). Each symbol represents an individual recipient. Horizontal lines in B and C indicate the median. Data are pooled from four independent experiments. *P < 0.05 and ****P < 0.0001 (Mann-Whitney test).