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. 2020 Jul 20;217(8):e20192336. doi: 10.1084/jem.20192336

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© 2020 Ruscher et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 5. — Reliance on TCR for IEL maintenance. (A) Representative plots of peripheral blood samples taken from TCR Cα^fl/fl (left plot, control) or UBC^CreERT2×TCR Cα^fl/fl mice (right plot) 7 d after administration of tamoxifen. The samples were surface stained for CD4 and CD8 and surface stained with one fluorochrome-conjugated antibody to TCRβ, then intracellularly stained with another. Shown are CD4⁻CD8⁻ cells (gray) and CD8⁺ T cells (black). (B–E) Frequency of surface TCRβ⁺ within total (surface and intracellular) TCRβ⁺ CD8αβ IELs (B) or CD8αα IELs (C) and numbers of total CD8αβ IELs (D) and CD8αα IELs (E) in the small intestine of TCR Cα^fl/fl (control) and UBC^CreERT2×TCR Cα^fl/fl (sample) mice. (F) Proposed model for CD8αα IEL establishment. Each symbol in B–E represents an individual mouse. Horizontal lines indicate the mean. Data are pooled from eight independent experiments. *P < 0.05 and ***P < 0.001 (ANOVA with Bonferroni post-test).