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. 2020 Jul 20;217(8):e20192336. doi: 10.1084/jem.20192336

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© 2020 Ruscher et al.

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Figure S2. — Labeling kinetics of time-stamped cells in the thymus. (A) Adult (solid black line) or 5–8-d-old (dashed line and solid pink line) stop-floxed tdTomato mice were intraperitoneally injected with tamoxifen (adult and 5–8 d old, solid pink line) or 4-OHT (5–8 d old, dashed line). The proportion of Tomato⁺ thymocytes was analyzed by flow cytometry 2, 5–6, and 8–10 d later. (B and C) 7-d-old stop-floxed tdTomato mice were injected with 4-OHT. (B) Plots showing Tomato label versus the maturation marker H-2K^b 2 d, 5 d, and 8 d after 4-OHT administration. (C) Cells within the Tomato^Int and Tomato^High fractions in A were plotted as CD4 versus CD8α. Data in A are combined from two independent experiments and show the mean ± SEM. Plots in B and C are representative of a minimum of two independent experiments.