Figure 1.

Prospective isolation and transcriptional profiling of CAFs from primary HGSOC samples. (A) Representative images of IF on five different HGSOC samples using pan-CK (CK; red) and CD49e (green) antibodies. Scale bar = 100 µm. (B) Representative FACS plots from six different patients showing the viable, CD45⁻CD31⁻ population stained for EpCAM and CD49e. (C) Representative images of cytospins made from isolated EpCAM⁺ or CD49e⁺ cells stained for pan-CK (red), vimentin (green), and Hoechst (blue). Scale bar = 50 µm. (D) Representative images of cytospins made from isolated EpCAM⁺ or CD49e⁺ cells stained for p53 (red) and Hoechst (blue). Scale bar = 50 µm. (E) Heatmap showing unsupervised hierarchical clustering of CD49e⁺ and EpCAM⁺ cells isolated directly from HGSOC tumor specimens and profiled by Illumina HT-12v4 microarrays. The EpCAM⁺ cells were separated into CD133⁺ and CD133⁻ fractions. The heatmap shows EpCAM⁺ populations from 12 patients and CD49e⁺ samples from 10 patients due to data being of poor quality from two of the CD49e⁺ samples. (F) GSEA of the genes differentially expressed in the CD49e⁺ versus EpCAM⁺ populations. The top 20 nonredundant gene sets for each population are shown. CREB, cyclic adenosine 3′,5′-monophosphate response element-binding protein; EGFR, epidermal growth factor receptor; FGFR, fibroblast growth factor receptor; NES, normalized enrichment score; NGF, nerve growth factor; PKA, protein kinase A; PLC phospholipase C; RHO, Ras homolog family member; TRKA, tropomyosin receptor kinase A.