Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 20;217(8):e20191094. doi: 10.1084/jem.20191094

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Hussain et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — FAP-high patients correspond to the mesenchymal molecular subtype and have worse clinical outcomes than FAP-low patients. (A) Heatmap of TCGA HGSOC data showing unsupervised clustering of patients using the FAP-high (FH) and FAP-low (FL) specific CAF gene lists. The TCGA molecular subtypes (Verhaak et al., 2013) are shown above the heatmap in blue. Patients expressing either 75% or 50% of the FAP-high signature genes are indicated in black and gray, respectively. Patients expressing either 75% or 50% of the FAP-low signature genes are indicated in red and pink, respectively. (B) GSEA comparing the FAP-high (left) and FAP-low (right) gene lists to the genes expressed in TCGA mesenchymal subtype. (C) The ABSOLUTE (left) and ESTIMATE (right) algorithms were applied to patients falling into the FAP-high (black), FAP-low (red), and “other” (blue) categories of patients selected using the more stringent 75% cutoff. (D) Kaplan–Meier survival curves of patients falling into the FAP-high (n = 80) and FAP-low (n = 30) groups (based on the 75% cutoff). Median progression-free survival was 1.2 yr for FAP-high and 2.1 yr for FAP-low patients; log-rank P value = 0.0204; hazard ratio (HR) = 1.89; 95% confidence interval (CI), 1.11–2.97. Median overall survival was 3.3 yr for FAP-high and 5.7 yr for FAP-low patients; log-rank P value = 0.0236, HR (95% CI) = 1.95 (1.11–3.24). (E) Kaplan–Meier progression-free survival curve of patients from our center that were profiled by FC for the proportion of CD49e⁺ CAFs that were positive for FAP staining. Patients were separated into FAP-high and FAP-low based on the median FAP expression. Log-rank P value = 0.041, HR (95% CI) = 2.28 (1.04–5.05). See Table S1 B for recurrence data.