Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 20;217(8):e20191094. doi: 10.1084/jem.20191094

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Hussain et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure S3. — Quantification of FAP and TCF21 in HGSOC specimens. (A) Low-power images of entire tissue sections showing the pan-CK staining in the red channel (top row) and the HALO software–generated classification of epithelial (red) and stromal (green) compartments. Blue regions are empty space. Scale bars = 1 mm. (B) Representative images of HGSOC specimens at higher magnification showing the epithelial pan-CK⁺ (red) and FAP⁺ or TCF21⁺ (green) staining and the corresponding mask generated by HALO software for quantification of the FAP or TCF21 signal within the stromal regions. Serial sections are shown for FAP versus TCF21 comparison. DAPI⁺ nuclei within the stroma are white, and FAP or TCF21⁺ signal in the green channel are overlayed with yellow. Scale bar = 500 µm. (C) Comparison of the %FAP-high cells in tumor tissue obtained from the primary site (ovary, n = 52) versus omental metastases (n = 10). *, P = 0.047, Student’s t test.