Fig. 9.

Focal abnormality of cortactin expression in the neural folds of p120fl/fl;Wnt1Cre mutant mice. a-l Immunofluorescence of p120ctn (left panels; nuclear counterstaining by DAPI) and cortactin (middle panels; overlay in right panels) on neural folds/tube in the hindbrain region of young embryos. Detection was on 9-somite (a-c) and 12–13 somite (g-i) control embryos, and on 7-somite (d-f) and 12–13 somite (j-l) mutant embryos. Green annotations point at positive signals; white annotations point at expression deficiency; arrows point at lateral sides of neural folds/tube; arrowheads point at the very tip of neural folds. In the control embryos (a-c, g-i), p120ctn and cortactin were coexpressed in neural folds/tube (arrows, arrowheads). In the mutant embryos (d-f, j-l), both p120ctn and cortactin were lost at the very tip of the neural folds (arrowheads), but the p120ctn-lacking lateral side of the neural folds retained cortactin positivity (arrows). Scale bars: 20 μm