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Binding properties of the 32A9 IgG antibody. ELISA to detect 32A9 binding activity (a) and affinity (b) to GPC3-hFc protein. Flow cytometry to detect the 32A9 binding signal (c) and affinity (d) to GPC3-positive cells. e ELISA to detect 32A9 binding specificity to human GPC3 and mouse GPC3. f ELISA to detect 32A9 binding specificity to GPC3 and GPC5. Values represent the mean ± SD, with ***p < 0.001
