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. 2020 Aug 3;20:366. doi: 10.1186/s12935-020-01461-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Prediction of the transcription factor(s) of the key genes and validation of transgelin-PARP1 interaction. a An illustration of the computational model for predicting the transcription factors of the key genes. b The prediction of the transcription factors of the key genes (partially illustrated). PARP1 protein is circled in a blue frame. c The recommendation degree of PARP1 as the transcription factor of the seven key genes downstream of transgelin. d The sequence of PARP1 protein. The red fonts represent the corresponding sequences of possible nuclear localization signal in PARP1 protein. e Immunoblotting analysis of transgelin and flag protein expression in RKO-TAGLN-FLAG, RKO-CTRL and wild type RKO cells, ****P < 0.0001. f Interaction between the transgelin-flag fusion protein and PARP1 was validated by co-immunoprecipitation. RKO-CTRL was the control group and normal rabbit IgG was used as the control antibody. g Immunofluorescence analysis of transgelin and PARP1 in RKO cells. The panels show transgelin immunostaining (green), PARP1 immunostaining (red), 4′,6-diamidino-2-phenylindole DNA staining (DAPI), and a merged image is indicated