Figure 6.

CLSM images of MCF-7 cells after (a, b) 3 h and (c, d) 24 h incubation with (a, c) 2 μm or (b, d) 700 nm peptide-free PMAA hydrogels particles; The cell nuclei and membranes were stained with DAPI (blue) and Wheat Germ Agglutinin, Alexa Fluor™ 555 Conjugate (WGA Alexa 555) (red), respectively, while hydrogels emit green fluorescence (FITC). Scale bar is 20 μm in all images. (e) MCF-7 cell internalization (%) of 2 μm and 700 nm peptide-free PMAA hydrogels as measured by flow cytometry. (*:p<0.05, #:p<0.01; data is represented as mean ± sd, n=2–4)