Table 2. Vero cells infected with PS-exosomes or PS-exosome depleted stool.
Virus | Sample | Time-point | Mean ± SEM | Significance |
---|---|---|---|---|
GII.4 Sydney | PS-exosomes | 0h | 1.00 ± 0.12 | – |
72h | 1.43 ± 0.12 | * | ||
PS-depleted | 0h | 1.00 ± 0.09 | – | |
72h | 1.94 ± 0.13 | **** | ||
GII.3 | PS-exosomes | 0h | 1.00 ± 0.08 | – |
72h | 1.42 ± 0.17 | * | ||
PS-depleted | 0h | 1.00 ± 0.09 | – | |
72h | 2.67 ± 0.28 | **** | ||
GII.4 Den Haag | stool | 0h | 1.01 ± 0.13 | – |
72h | 1.28 ± 0.17 | ns | ||
PS-exosomes | 0h | 1.00 ± 0.14 | – | |
72h | 1.22 ± 0.18 | ns | ||
PS-depleted | 0h | 0.96 ± 0.07 | – | |
72h | 0.99 ± 0.13 | ns |
qRT-PCR analysis after a 72h infection (MOI = 0.1). Mean fold-changes over 0h time-points displayed. Significant differences between 0h and 72h time-points were calculated. Magnetic bead partitioning was used to isolate PS-exosomes and generate PS-depleted stool samples. Data represent n = 3 ± SEM.